Digital lensless holographic microscopy: numerical simulation and reconstruction with ImageJ
Por:
Trujillo, Carlos, Piedrahita-Quintero, Pablo, Garcia-Sucerquia, Jorge
Publicada:
1 jul 2020
Resumen:
The description and validation of an ImageJ open-source plugin to
numerically simulate and reconstruct digital lensless holographic
microscopy (DLHM) holograms are presented. Two modules compose the
presented plugin: the simulation module implements a discrete version of
the Rayleigh-Somerfield diffraction formula, which allows the user to
directly build a simulated hologram from a known phase and/or amplitude
object by just introducing the geometry parameters of the simulated
setup; the plugin's reconstruction module implements a discrete version
of the Kirchhoff-Helmholtz diffraction integral, thus allowing the user
to reconstruct DLHM holograms by introducing the parameters of the
acquisition setup and the desired reconstruction distance. The plugin
offers the two said modules within the robust environment provided by a
complete set of built-in tools for image processing available in ImageJ.
While the simulation module has been validated through the evaluation of
the forecasted lateral resolution of a DLHM setup in terms of the
numerical aperture, the reconstruction module is tested by means of
reconstructing experimental DLHM holograms of biological samples. (C)
2020 Optical Society of America
Filiaciones:
Trujillo, Carlos:
Univ EAFIT, Appl Opt Grp, Phys Sci Dept, Sch Sci, Medellin, Colombia
Piedrahita-Quintero, Pablo:
Univ Nacl Colombia, Sch Phys, Sede Medellin, AA 3840, Medellin 050034, Colombia
Garcia-Sucerquia, Jorge:
Univ Nacl Colombia, Sch Phys, Sede Medellin, AA 3840, Medellin 050034, Colombia
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