Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
Por:
CORREA, JAVIER
Publicada:
1 jul 2018
Resumen:
Abstract The cultivation of sugarcane represents an important part in
the Colombian economy due to the diverse applications in industries like
liquor, food, paper and biofuels. Sugarcane worldwide production is
affected by the presence of phytopathogenic agents, mainly filamentous
fungi such as Physalospora tucumanensis (red rot disease) and Fusarium
spp. To date in Colombia, Pokka boheng disease whose causal agent is the
fungus Fusarium verticillioides, has not been reported, which is why it
is necessary to identify appropriately this microorganism, responsible
for losses in productivity and food contamination. In order to isolate
and identify the infectious agent from symptomatic tissues, disinfection
and culture in liquid and solid culture mediums were performed in malt
extract (2%) and yeast extract (0.2%) both liquid and solid, for 7 days.
After several replicates in agar plate, a purification was made along
with a morpho- logical characterization based on the shape and color of
the mycelium, as well as the type of spores generated. Additionally, the
genetic material was extracted and gene markers (ITS, Elongation Factor
1-a (EF) and ß- Tubulin (Btub)) were amplified by PCR. Then, DNA
sequencing was used to obtain the data to make a phylogenetic
reconstruction by probabilistic methods (Maximum Likelihood and Bayesian
Inference). The isolated strain, named as EA-FP0013 was located in the
Fujikuroi complex group, with high probable identity to Fusarium
verticillioides. Thus, early and species-specific identification of
these fungal isolates by molecular methods may allow the timely
diagnosis of emerging pathophysiological diseases of interest in the
region, and thus propose the respective control strategies.
Filiaciones:
CORREA, JAVIER:
Universidad Eafit
|