Fengycin C produced by Bacillus subtilis EA-CB0015.
Por:
Villegas-Escobar, Valeska, Ceballos, Isabel, Mira, John J., Edith Argel, Luz, Orduz Peralta, Sergio, Romero-Tabarez, Magally
Publicada:
26 abr 2013
Ahead of Print:
5 mar 2013
Resumen:
Bacillus subtilis EA-CB0015 was isolated from the phyllosphere of a banana plant and tested for its potential to produce bioactive compounds against Mycosphaerella fijiensis. Using a dual plate culture technique the cell-free supernatant of B. subtilis EA-CB0015 produced inhibition values of 89 +/- 1%. The active compounds were purified by solid-phase extraction and HPLC, and their primary structures determined using mass spectrometry and amino acid analysis. A new fengycin isoform, fengycin C, with the amino acid sequence Glu-Orn-Tyr-Thr-Glu-Val-Pro-Gln-Thr-Ile was isolated. The peptidic moiety differs from fengycin B at position 9 and from fengycin A at positions 6 and 9. The beta-hydroxy fatty acyl chain is connected to the N-terminal of the decapeptide and can be saturated or unsaturated, ranging from 14 to 18 carbons. The C-terminal residue of the peptidic moiety is linked to the tyrosine residue at position 3, forming the branching point of the acyl peptide and the eight-membered cyclic lactone.
Filiaciones:
Villegas-Escobar, Valeska:
Univ EAFIT, Dept Ingn Proc, Grp GIPAB, Medellin, Colombia
Ceballos, Isabel:
Univ Nacl Colombia, Inst Biotecnol, Bogota, Colombia
Mira, John J.:
Ctr Invest Banano, Carepa, Colombia
Edith Argel, Luz:
Ctr Invest Banano, Carepa, Colombia
Orduz Peralta, Sergio:
Univ Nacl Colombia, Medellin, Colombia
Romero-Tabarez, Magally:
Univ Nacl Colombia, Medellin, Colombia
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