Mitochondrial genome characterization of Tecia solanivora (Lepidoptera: Gelechiidae) and its phylogenetic relationship with other lepidopteran insects
Por:
Ramírez-Ríos, V., Franco-Sierra, N.D., Alvarez, J.C., Saldamando-Benjumea, C.I., Villanueva, D.
Publicada:
1 may 2016
Categoría:
Genetics
Resumen:
The complete mitogenome of the potato tuber moth Tecia solanivora
(Lepidoptera: Gelechiidae) was sequenced, annotated, characterized and
compared with 140 species of the order Lepidoptera. The circular genome
is 15,251 bp, containing 37 genes (13 protein-coding genes (PCGs), two
rRNA genes, 22 tRNA genes and an A + T-rich region). The gene
arrangement was identical to other lepidopteran mitogenomes but
different from the ancestral arrangement found in most insects for the
tRNA-Met gene (A + T-region, tRNA-I, tRNA-Q, tRNA-M). The mitogenome of
T. solanivora is highly A + T-biased (78.2%) and exhibits negative AT-
and GC-skews. All PCGs are initiated by canonical ATN start codons,
except for Cytochrome Oxidase subunit 1 (COI), which is initiated by
CGA. Most PCGs have a complete typical stop codon (TAA). Only NAD1 has a
TAG stop codon and the COII and NAD5 genes have an incomplete stop codon
consisting of just a T. The A + T-rich region is 332 bp long and
contains common features found in lepidopteran mitogenomes, including
the `ATAGA' motif, a 17 bp poly (T) stretch and a (AT)(8) element
preceded by the `ATTTA' motif. Other tandem repeats like (TAA)(4) and
(TAT), were found, as well as (T)(6) and (A)(10) mononucleotide repeat
elements. Finally, this mitogenome has 20 intergenic spacer regions. The
phylogenetic relationship of T. solanivora with 28 other lepidopteran
families (12 superfamilies) showed that taxonomic classification by
morphological features coincides with the inferred phylogeny. Thus, the
Gelechiidae family represents a monophyletic group, suggesting that T.
solanivora and Pectinophora gossypiella have a recent common ancestor.
(C) 2016 Elsevier B.V. All rights reserved.
Filiaciones:
Ramírez-Ríos, V.:
Departamento de Ciencias Biológicas, Escuela de Ciencias, Universidad EAFIT, Medellín, Antioquia, Colombia
Franco-Sierra, N.D.:
Departamento de Ciencias Biológicas, Escuela de Ciencias, Universidad EAFIT, Medellín, Antioquia, Colombia
Alvarez, J.C.:
Departamento de Ciencias Biológicas, Escuela de Ciencias, Universidad EAFIT, Medellín, Antioquia, Colombia
Saldamando-Benjumea, C.I.:
Escuela de Biociencias, Facultad de Ciencias, Universidad Nacional de Colombia, sede Medellín, Antioquía, Colombia
Villanueva, D.:
Departamento de Ciencias Biológicas, Escuela de Ciencias, Universidad EAFIT, Medellín, Antioquia, Colombia
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