Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
Por:
Posada, L.F., Alvarez, J.C., Hu, C.-H., de-Bashan, L.E., Bashan, Y.
Publicada:
1 sep 2016
Resumen:
Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB)
of many crops and are used as inoculants. PGPB colonization is an
important trait for success of a PGPB on plants. A specific probe, based
on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to
distinguishing, by fluorescence in situ hybridization (FISH), between
this species and the closely related Bacillus amyloliquefaciens. The
selected target for the probe was between nucleotides 465 and 483 of the
gene, where three different nucleotides can be identified. The designed
probe successfully hybridized with several strains of Bacillus subtilis,
but failed to hybridize not only with B. amyloliquefaciens, but also
with other strains such as Bacillus altitudinis, Bacillus cereus,
Bacillus gibsonii, Bacillus megaterium, Bacillus pumilus; and with the
external phylogenetic strains Azospirillum brasilense Cd, Micrococcus
sp. and Paenibacillus sp. The results showed the specificity of this
molecular probe for B. subtilis. (C) 2016 Elsevier B.V. All rights
reserved.
Filiaciones:
Posada, L.F.:
Department of Process Engineering, Universidad EAFIT, Cra 49 #7 sur-50, Medellín, Colombia
Alvarez, J.C.:
Departament of Biological Sciences, Universidad EAFIT, Cra 49 #7 sur-50, Medellín, Colombia
Hu, C.-H.:
Department of Entomology and Plant Pathology, Auburn University, 301 Funchess Hall, Auburn, AL, United States
de-Bashan, L.E.:
The Bashan Institute of Science, 1730 Post Oak Ct.AL, United States
Department of Entomology and Plant Pathology, Auburn University, 301 Funchess Hall, Auburn, AL, United States
Environmental Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Av. IPN 195, La Paz, B.C.S., Mexico
Bashan, Y.:
The Bashan Institute of Science, 1730 Post Oak Ct.AL, United States
Department of Entomology and Plant Pathology, Auburn University, 301 Funchess Hall, Auburn, AL, United States
Environmental Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Av. IPN 195, La Paz, B.C.S., Mexico
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