Phylogenetic and pathogenic variability of strains of Ralstonia solanacearum causing moko disease in Colombia
Por:
Ramirez, M., Moncada, R. N., Villegas-Escobar, V., Jackson, R. W., Ramirez, C. A.
Publicada:
1 feb 2020
Resumen:
Moko disease, caused by the bacterium Ralstonia solanacearum, is one of
the most devastating diseases of Musa spp. in Colombia, where banana and
plantain are major crops. The disease epidemiology is poorly understood
and little is known about the diversity of the bacterial populations
associated with this disease. This study assessed the diversity,
phylogenetic relationship and pathogenicity of R. solanacearum strains
associated with moko disease in Colombia. For this, the genetic
diversity of 65 isolates obtained from four banana/plantain-growing
regions was evaluated by using multiplex PCR and analysing the partial
sequences of the mutS, rplB and egl genes. These analyses revealed that
all the strains belonged to the R. solanacearum phylotype II, sequevars
4 and 6. In addition, the phylogenetic analysis assorted the strains
into three subgroups, which matched the region of isolation: (i) central
region (i.e. Eastern plains and Andes, IIB/4); (ii) northwest (i.e.
Uraba and a few strains from Magdalena, IIB/4); and (iii) north coast
(Magdalena and a few strains from Uraba, IIA/6). In addition, this
evolutionary pattern was associated with pathogenicity, as 63 of the 65
isolates caused wilting of banana and plantain plants under greenhouse
conditions, whilst only 32, those isolated from the central region,
caused such symptoms in tomato plants. In conclusion, this study shows
that banana and plantain crops in Colombia foster genetically diverse
strains of R. solanacearum that belong to at least three different
genetic groups, which show biogeographic and host range association.
Filiaciones:
Ramirez, M.:
Univ Antioquia, Inst Biol, Calle 67 53-108, Medellin, Colombia
Moncada, R. N.:
Univ EAFIT, Dept Biol Sci, Carrera 49 7 Sur 50, Medellin, Colombia
Villegas-Escobar, V.:
Univ EAFIT, Dept Biol Sci, Carrera 49 7 Sur 50, Medellin, Colombia
Jackson, R. W.:
Univ Reading, Sch Biol Sci, Reading RG6 6AJ, Berks, England
Ramirez, C. A.:
Univ Antioquia, Inst Biol, Calle 67 53-108, Medellin, Colombia
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